Pretreatment with cisplatin enhances E7-specific CD8+ T-Cell-mediated antitumor immunity induced by DNA vaccination.

Effect of LIGHT Adjuvant on Kinetics of T-Cell Responses Induced by HSV-1 DNA Immunization

Background: Studies on efficacy of various vaccines that prevent or reduce the primary and recurrent HSV-1 infection have demonstrated the importance of cellular immunity for protection against the infection. We previously used DNA vaccination to induce cellular immunity against HSV-1 infection in mice. Objective: The aim of our study was to evaluate the effect of LIGHT, a member of TNF super f...

Depletion of platelets enhances therapeutic antitumor effects generated by therapeutic DNA vaccine

It has been well established that platelets are associated with tumor progression and metastasis as they can support angiogenesis and prevent hemorrhage. As such, platelets may be an ideal target for enhancing anticancer immunotherapies. Thus, we hypothesized that inducing thrombocytopenia would increase the leakiness of tumor vasculature thereby facilitating the effective delivery of antigen-s...

Strong Immune Responses Induced by a DNA Vaccine Containing HPV16 Truncated E7 C-terminal Linked to HSP70 Gene

Background: Vaccines capable of controlling tumor virus based infections are found difficult to develop due to the consistence latent infection in the host. DNA vaccines are attractive tools for the development of HPV vaccines and inducing antigen-specific immunity owing to the stability, simplicity of delivery, safety and cost effectiveness. However, there is a need to increase their potency b...

Antitumor Immunity Induced by Genetic Immunization with Chitosan Nanoparticle Formulated Adjuvanted for HPV-16 E7 DNA Vaccine

Therapeutic synergy of human papillomavirus E7 subunit vaccines plus cisplatin in an animal tumor model: causal involvement of increased sensitivity of cisplatin-treated tumors to CTL-mediated killing in therapeutic synergy.

PURPOSE The goal of this study was to investigate the therapeutic potentials of combining chemotherapy with human papillomavirus (HPV) E7 subunit vaccines in an animal tumor model and to determine the underlying therapeutic mechanisms. EXPERIMENTAL DESIGN Animals bearing HPV E6/E7-expressing tumors were treated intratumorally with a selected cytotoxic drug, cisplatin, twice at 1-week interval...